Screening and verification of species-specific 16S rRNA fragments for the identification of S.aureus

• 1、Chemisitry and Chemical Engineering School, Hunan University, Changsha, 410082

Abstract：S.aureus is a widely distributed pathogen that causes many infectious diseases. Thus, the identi?cation and quantitation of S. aureus have become a crucial point in medical diagnosis, food safety, environmental hygiene, and drug discovery. However, the lack of ideal targets with high abundance, good intraspecies universality, good interspecies specificity, and easy measurement has affected the accuracy of S. aureus detection methods. In order to obtain the ideal target, this article uses 16S rRNA with high abundance in the bacteria as the research object, Using the intra-species universality, interspecies specificity, hybridization efficiency and anti-interference ability as evaluation indicators, the 16S rRNA sequence fragment targets that can identify S. aureus species were selected and verified by PCR and electrophoresis technology. In summary, the ideal method for bacterial species identification can be obtained by using the constructed method, and this method has broad application prospects in the design of bacterial species identification targets.

Keywords： S. aureus 16S rRNA Identification target